C6 Configuration: The C6 contains two collinear lasers and is arranged in such a way that four detectors all simultaneously measure light scatter. The detector
1.3 BD Accuri C6 Flow Cytometer Specifications Table 1-3. BD Accuri C6 Flow Cytometer Specifications. Laser Excitation. 488 nm (rated at 20,000 hr life) 640 nm (rated at 20,000 hr life) Laser Profile. 10 x 75 µm . Laser Power . 14.7 mW 640 nm Diode Red Laser
Do not open the Laser Head System consists of: BD Accuri C6 Flow Cytometer Bottle rack Sheath fluid bottle Configuration and Setup: Number of Lasers: 2 Laser type: 14.7mW 640nm Mar 7, 2015 The BD Accuri C6 is equipped with (i) two lasers which will allow for blue (488 nm) and red (633 nm) excitation, (ii) two light scatter detectors reagent containers, complimentary BD Accuri C6 Plus flow cytometer starter kit and 2 month maintenance kit, and one- year warranty Max Colors 4 Max Lasers 2 . Minimize spillover. • Spread anfgens across lasers Laser and detectors: the type and number of laser and detectors dictate whether the BD Accuri C6: 488- ‐ Jul 2, 2020 With 5 lasers and 28 different fluorescent detectors the Symphony can The Accuri C6 is based in the medical school as is our most smallest Mar 9, 2021 The system has the maximum of 6 lasers installed (375 nm, 405 nm, 488 6- parameter analysis on an ACCURI C6 Flow Cytometer Analyzer Laser and filters BD Accuri™ C6 Flow Cytometer Optical Filter Guide. Software. BD CSamplerTM software based on BD AccuriTM C6 software Accuri™ C6. iCyt™.
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The BD Accuri C6 flow cytometer is a small benchtop anlyser equipped with 2 lasers, blue (488nm) and red (633nm) and is capable of 4 colour, 6 parameter analysis (4 colour + FSC + SSC). The Accuri C6 has a fixed optical alignment and pre-optmized detector settings and data is collected over a 7 decade dynamic range, therefore adjustment of detector voltages is not necessary. Suggested Compensation Values for the BD Accuri C6. Compensation Controls – Beads Advantages of compensation beads – Easy to use – Do not require cells 29 FITC PE. BD Accuri™ C6 Software Sample Grid Cytometer Status Standard C6 configuration Selectable Lasers: 2-blue 2-red. Want to build a panel on the Accuri C6 using FluoroFinder? Click here! The BDAccuri C6 is the flow cytometer (analyzer) in the flow core. The Accuri is a small, bench-top cytometer housing 2 light scatter detectors, 2 lasers, and 4 fluorescence detectors that enable the detection of up to four fluorochromes.
Troubleshooting for BYU Life Sciences' Accuri C6 Flow CytometerNarrated by Dr. Donald BreakwellFilmed and edited by Gabriel Goulding
4 This filter transmits red laser light, which may damage the detector. Use only when the instrument is in the 4-blue configuration of the Selectable Lasers Module. 5Standard filter in alternate position.
BD Accuri C6 with sample volumes as low as 300 μL (assuming a volume run limit of 10 μL) in BD Falcon™ 12 x 75-mm tubes. The maximum recommended sample volume in a 12 x 75-mm tube is 2 mL. Never acquire more than 750 μ L from a single tube using the Medium fluidics setting, or …
Optional filters and the Selectable Laser Module expand the available fluorochrome combinations.
4 This filter transmits red laser light, which may damage the detector. Use only when the instrument is in the 4-blue configuration of the Selectable Lasers …
BD Accuri C6 Plus flow cytometer With its compact 11 x 14.75 x 16.5-in footprint, light weight of 30 lb and operational simplicity, the BD Accuri C6 Plus flow cytometer supports a wide array of applications including immunology, cell and cancer biology, plant and microbiology, and industrial applications. BD accuri C6 cytometer manual Getting ready Ensure that the fluid levels in the Sheath, Cleaning, and Decontamination bottles are sufficient to cover the inlet tubing and that there are no kinked fluidic lines. Turn on the computer and log in with your UCF info. Start the Accuri CSampler software. Accuri C6 is a 4 color (2 lasers) instrument, Facs Verse may be up to 8 (3 lasers) color.
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Beginning of dialog window. BD Accuri C6 Plus flow cytometer. With its compact 11 x 14.75 x 16.5-in footprint, light weight of 30 lb and operational simplicity, the BD Accuri C6 Plus flow cytometer supports a wide array of applications including immunology, cell and cancer biology, plant and microbiology, and industrial applications.
SIMPLE.
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BD Accuri C6 Flow Cytometer Instrument Manual 2 7820018-01 Rev-2 Table 1-3. BD Accuri C6 Flow Cytometer Specifications Laser Excitation 488 nm (rated at 20,000 hr life) 640 nm (rated at 20,000 hr life) Laser Profile 10 x 75 µm Laser Power 14.7 mW 640 nm Diode Red Laser 20 mW 488 nm Solid State Blue Laser
The default configuration of the BD Accuri ® C6 is similar to the FACSCalibur-2 (3-Blue 1-Red) with filters optimized for the detection of fluorochromes such as FITC, PE, PerCP, and APC. 2.5. Laser Precautions The C6 Flow Cytometer contains a solid state 488nm and a 640nm diode laser under the outer and secondary covers.
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BD accuri C6 cytometer manual Getting ready Ensure that the fluid levels in the Sheath, Cleaning, and Decontamination bottles are sufficient to cover the inlet tubing and that there are no kinked fluidic lines. Turn on the computer and log in with your UCF info. Start the Accuri CSampler software.
Prover analyserades med användning Rav4 2018 owners manual nihongo · Apple tv manual dns settings · Bd accuri c6 software manual · Jac j3 2011 gasolina 4p manual · Pentax optio z10 manual Cykelcykeln analyserades i Accuri C6-flödescytometern (BD Biosciences, San Tuning Quantum Efficiency of Random Lasers - Intrinsic Stokes-Shift and Gain användning av en flödescytometer (BD Accuri ™ C6; BD Biosciences, USA). GFP-fluorescens exciterades av en 488 nm laser och övervakades genom ett FL1 The BD Accuri™ C6 Plus is equipped with a blue and red laser, two light scatter detectors, and four fluorescence detectors with optical filters optimized for the detection of many popular fluorochromes, including FITC, PE, PerCP-Cy™5.5, and APC, as well as newer polymer dyes such as BD Horizon Brilliant™ Blue 515.
BD Accuri C6 Plus flow cytometer, detectors FL1, FL2, and FL3 read fluorescence emissions from the blue laser, while detector FL4 reads emissions from the red laser (3-blue/1-red). By installing the optional Selectable Laser Module (Cat. No. 653126), researchers can operate the system in 2-blue/2-red and 4-blue configurations, which significantly
Figure 1-1. The BD Accuri C6 Flow Cytometer System The BD Accuri C6 flow cytometer system includes BD Accuri C6 Software for controlling the instrument 2016-03-01 BD Accuri C6 with sample volumes as low as 300 μL (assuming a volume run limit of 10 μL) in BD Falcon™ 12 x 75-mm tubes. The maximum recommended sample volume in a 12 x 75-mm tube is 2 mL. Never acquire more than 750 μ L from a single tube using the Medium fluidics setting, or … Flow Cytometry within Reach The BD Accuri™ C6 is a personal flow cytometer that brings cell analysis within reach by being easy to use, simple to maintain, and affordable.
BD Accuri C6 Plus flow cytometer.